Construct: shRNA TRCN0000083686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057176.2-958s1c1
DNA Barcode:
CAAGACTTTGCCCTGATTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BSND (7809)
Gene Description:
barttin CLCNK type accessory beta subunit
Transcript:
RefSeq NM_057176.2 (NON-CURRENT)
Match location:
Position 958 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGACTTTGCCCTGATTGAT
Hairpin Sequence:
5'-CCGG-CAAGACTTTGCCCTGATTGAT-CTCGAG-ATCAATCAGGGCAAAGTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGACTTTGCCCTGATTGATCTCGAGATCAATCAGGGCAAAGTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGACTTTGCCCTGATTGATCTCGAGATCAATCAGGGCAAAGTCTTG-3'

Other clones with same target sequence:

(none)