Construct: shRNA TRCN0000083702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000561.2-585s1c1
DNA Barcode:
CTTCCCAAATCTGAAGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSTM1 (2944)
Gene Description:
glutathione S-transferase mu 1
Transcript:
RefSeq NM_000561.2 (NON-CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCCAAATCTGAAGGACTT
Hairpin Sequence:
5'-CCGG-CTTCCCAAATCTGAAGGACTT-CTCGAG-AAGTCCTTCAGATTTGGGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCCAAATCTGAAGGACTTCTCGAGAAGTCCTTCAGATTTGGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCCAAATCTGAAGGACTTCTCGAGAAGTCCTTCAGATTTGGGAAG-3'

Other clones with same target sequence:

  1. TRCN0000178757