Construct: shRNA TRCN0000083747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021980.3-768s1c1
DNA Barcode:
GCACGGCATTGTCTAAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OPTN (10133)
Gene Description:
optineurin
Transcript:
RefSeq NM_021980.3 (NON-CURRENT)
Match location:
Position 768 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGGCATTGTCTAAATATA
Hairpin Sequence:
5'-CCGG-GCACGGCATTGTCTAAATATA-CTCGAG-TATATTTAGACAATGCCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGGCATTGTCTAAATATACTCGAGTATATTTAGACAATGCCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGGCATTGTCTAAATATACTCGAGTATATTTAGACAATGCCGTGC-3'

Other clones with same target sequence:

(none)