Construct: shRNA TRCN0000083814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001317.3-279s1c1
DNA Barcode:
CAGGAGTTTGAAGAAACCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSH1 (1442)
Gene Description:
chorionic somatomammotropin hormone 1
Transcript:
RefSeq NM_001317.3 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGTTTGAAGAAACCTAT
Hairpin Sequence:
5'-CCGG-CAGGAGTTTGAAGAAACCTAT-CTCGAG-ATAGGTTTCTTCAAACTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGTTTGAAGAAACCTATCTCGAGATAGGTTTCTTCAAACTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGTTTGAAGAAACCTATCTCGAGATAGGTTTCTTCAAACTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000158524