Construct: shRNA TRCN0000084033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015087.3-2124s1c1
DNA Barcode:
GCCTTATGAAATGGATGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPART (23111)
Gene Description:
spartin
Transcript:
RefSeq NM_015087.3 (NON-CURRENT)
Match location:
Position 2124 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTATGAAATGGATGAAAT
Hairpin Sequence:
5'-CCGG-GCCTTATGAAATGGATGAAAT-CTCGAG-ATTTCATCCATTTCATAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTATGAAATGGATGAAATCTCGAGATTTCATCCATTTCATAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTATGAAATGGATGAAATCTCGAGATTTCATCCATTTCATAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000300009