Construct: shRNA TRCN0000084110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173859.1-430s1c1
DNA Barcode:
GCTAATATGCAGTCAGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BPIFA4P (317716)
Gene Description:
BPI fold containing family A member 4, pseudogene
Transcript:
RefSeq NM_173859.1 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATATGCAGTCAGATATA
Hairpin Sequence:
5'-CCGG-GCTAATATGCAGTCAGATATA-CTCGAG-TATATCTGACTGCATATTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATATGCAGTCAGATATACTCGAGTATATCTGACTGCATATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATATGCAGTCAGATATACTCGAGTATATCTGACTGCATATTAGC-3'

Other clones with same target sequence:

(none)