Construct: shRNA TRCN0000084215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029834.1-681s1c1
DNA Barcode:
GAAGAAGAATTGCTGCTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r12c (232807)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 12C
Transcript:
RefSeq NM_029834.1 (NON-CURRENT)
Match location:
Position 681 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAGAATTGCTGCTTCAT
Hairpin Sequence:
5'-CCGG-GAAGAAGAATTGCTGCTTCAT-CTCGAG-ATGAAGCAGCAATTCTTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAGAATTGCTGCTTCATCTCGAGATGAAGCAGCAATTCTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAGAATTGCTGCTTCATCTCGAGATGAAGCAGCAATTCTTCTTC-3'

Other clones with same target sequence:

(none)