Construct: shRNA TRCN0000084216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029834.1-411s1c1
DNA Barcode:
GATGAGAACCTGGAAGTCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r12c (232807)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 12C
Transcript:
RefSeq NM_029834.1 (NON-CURRENT)
Match location:
Position 411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGAACCTGGAAGTCGTT
Hairpin Sequence:
5'-CCGG-GATGAGAACCTGGAAGTCGTT-CTCGAG-AACGACTTCCAGGTTCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGAACCTGGAAGTCGTTCTCGAGAACGACTTCCAGGTTCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGAACCTGGAAGTCGTTCTCGAGAACGACTTCCAGGTTCTCATC-3'

Other clones with same target sequence:

(none)