Construct: shRNA TRCN0000084220

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133346.1-2467s1c1
DNA Barcode:
GCACATTGCTGTCCTGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asb6 (72323)
Gene Description:
ankyrin repeat and SOCS box-containing 6
Transcript:
RefSeq NM_133346.1 (NON-CURRENT)
Match location:
Position 2467 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084220)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATTGCTGTCCTGAGAAA
Hairpin Sequence:
5'-CCGG-GCACATTGCTGTCCTGAGAAA-CTCGAG-TTTCTCAGGACAGCAATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATTGCTGTCCTGAGAAACTCGAGTTTCTCAGGACAGCAATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATTGCTGTCCTGAGAAACTCGAGTTTCTCAGGACAGCAATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000316006