Construct: shRNA TRCN0000084227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026496.1-1907s1c1
DNA Barcode:
CCTGGTCAACATGGATGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grhl2 (252973)
Gene Description:
grainyhead-like 2 (Drosophila)
Transcript:
RefSeq NM_026496.3 (NON-CURRENT)
Match location:
Position 1907 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCAACATGGATGACAA
Hairpin Sequence:
5'-CCGG-CCTGGTCAACATGGATGACAA-CTCGAG-TTGTCATCCATGTTGACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCAACATGGATGACAACTCGAGTTGTCATCCATGTTGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCAACATGGATGACAACTCGAGTTGTCATCCATGTTGACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000316009