Construct: shRNA TRCN0000084266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172937.2-3165s1c1
DNA Barcode:
GCAGGCATTCACATCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shprh (268281)
Gene Description:
SNF2 histone linker PHD RING helicase
Transcript:
RefSeq NM_172937.2 (NON-CURRENT)
Match location:
Position 3165 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCATTCACATCATTAAA
Hairpin Sequence:
5'-CCGG-GCAGGCATTCACATCATTAAA-CTCGAG-TTTAATGATGTGAATGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCATTCACATCATTAAACTCGAGTTTAATGATGTGAATGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCATTCACATCATTAAACTCGAGTTTAATGATGTGAATGCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000288210