Construct: shRNA TRCN0000084271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009553.2-2126s1c1
DNA Barcode:
GCACCTGAAAGAGAAGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zscan2 (22691)
Gene Description:
zinc finger and SCAN domain containing 2
Transcript:
RefSeq NM_009553.2 (CURRENT)
Match location:
Position 2126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTGAAAGAGAAGCTTTA
Hairpin Sequence:
5'-CCGG-GCACCTGAAAGAGAAGCTTTA-CTCGAG-TAAAGCTTCTCTTTCAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTGAAAGAGAAGCTTTACTCGAGTAAAGCTTCTCTTTCAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTGAAAGAGAAGCTTTACTCGAGTAAAGCTTCTCTTTCAGGTGC-3'

Other clones with same target sequence:

(none)