Construct: shRNA TRCN0000084325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033322.2-434s1c1
DNA Barcode:
GCCTAAACTTGTTCCAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lztfl1 (93730)
Gene Description:
leucine zipper transcription factor-like 1
Transcript:
RefSeq NM_033322.2 (CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAAACTTGTTCCAATTAA
Hairpin Sequence:
5'-CCGG-GCCTAAACTTGTTCCAATTAA-CTCGAG-TTAATTGGAACAAGTTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAAACTTGTTCCAATTAACTCGAGTTAATTGGAACAAGTTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAAACTTGTTCCAATTAACTCGAGTTAATTGGAACAAGTTTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000302140