Construct: shRNA TRCN0000084366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023672.2-1506s1c1
DNA Barcode:
CTCCTTCCAGAACGACAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssbp3 (72475)
Gene Description:
single-stranded DNA binding protein 3
Transcript:
RefSeq NM_023672.2 (CURRENT)
Match location:
Position 1506 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTTCCAGAACGACAATTA
Hairpin Sequence:
5'-CCGG-CTCCTTCCAGAACGACAATTA-CTCGAG-TAATTGTCGTTCTGGAAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTTCCAGAACGACAATTACTCGAGTAATTGTCGTTCTGGAAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTTCCAGAACGACAATTACTCGAGTAATTGTCGTTCTGGAAGGAG-3'

Other clones with same target sequence:

  1. TRCN0000288156