Construct: shRNA TRCN0000084383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053123.3-3656s1c1
DNA Barcode:
GCTTTGAAAGAGAACCTAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarca1 (93761)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Transcript:
RefSeq NM_053123.3 (NON-CURRENT)
Match location:
Position 3656 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGAAAGAGAACCTAGAT
Hairpin Sequence:
5'-CCGG-GCTTTGAAAGAGAACCTAGAT-CTCGAG-ATCTAGGTTCTCTTTCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGAAAGAGAACCTAGATCTCGAGATCTAGGTTCTCTTTCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGAAAGAGAACCTAGATCTCGAGATCTAGGTTCTCTTTCAAAGC-3'

Other clones with same target sequence:

(none)