Construct: shRNA TRCN0000084388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015826.2-1312s1c1
DNA Barcode:
CGGCAGGGTTTGTTGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dmrt1 (50796)
Gene Description:
doublesex and mab-3 related transcription factor 1
Transcript:
RefSeq NM_015826.2 (NON-CURRENT)
Match location:
Position 1312 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAGGGTTTGTTGTTATTT
Hairpin Sequence:
5'-CCGG-CGGCAGGGTTTGTTGTTATTT-CTCGAG-AAATAACAACAAACCCTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAGGGTTTGTTGTTATTTCTCGAGAAATAACAACAAACCCTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAGGGTTTGTTGTTATTTCTCGAGAAATAACAACAAACCCTGCCG-3'

Other clones with same target sequence:

(none)