Construct: shRNA TRCN0000084415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153787.1-565s1c1
DNA Barcode:
GCCGGTCATATAGATCCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bclaf1 (72567)
Gene Description:
BCL2-associated transcription factor 1
Transcript:
RefSeq NM_153787.1 (NON-CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGTCATATAGATCCTCTA
Hairpin Sequence:
5'-CCGG-GCCGGTCATATAGATCCTCTA-CTCGAG-TAGAGGATCTATATGACCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGTCATATAGATCCTCTACTCGAGTAGAGGATCTATATGACCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGTCATATAGATCCTCTACTCGAGTAGAGGATCTATATGACCGGC-3'

Other clones with same target sequence:

  1. TRCN0000331806