Construct: shRNA TRCN0000084422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174852.2-1161s1c1
DNA Barcode:
CCTGAACCGAATCCACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf12 (268448)
Gene Description:
PHD finger protein 12
Transcript:
RefSeq NM_174852.2 (NON-CURRENT)
Match location:
Position 1161 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAACCGAATCCACAAGAA
Hairpin Sequence:
5'-CCGG-CCTGAACCGAATCCACAAGAA-CTCGAG-TTCTTGTGGATTCGGTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAACCGAATCCACAAGAACTCGAGTTCTTGTGGATTCGGTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAACCGAATCCACAAGAACTCGAGTTCTTGTGGATTCGGTTCAGG-3'

Other clones with same target sequence:

(none)