Construct: shRNA TRCN0000084450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178661.2-1321s1c1
DNA Barcode:
GTCTTGTTCAACTGAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Creb3l2 (208647)
Gene Description:
cAMP responsive element binding protein 3-like 2
Transcript:
RefSeq NM_178661.2 (NON-CURRENT)
Match location:
Position 1321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTGTTCAACTGAGAACTT
Hairpin Sequence:
5'-CCGG-GTCTTGTTCAACTGAGAACTT-CTCGAG-AAGTTCTCAGTTGAACAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTGTTCAACTGAGAACTTCTCGAGAAGTTCTCAGTTGAACAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTGTTCAACTGAGAACTTCTCGAGAAGTTCTCAGTTGAACAAGAC-3'

Other clones with same target sequence:

  1. TRCN0000301786