Construct: shRNA TRCN0000084509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173738.2-787s1c1
DNA Barcode:
CCTGGTAACAAAGTCCACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp940 (233057)
Gene Description:
zinc finger protein 940
Transcript:
RefSeq NM_173738.2 (CURRENT)
Match location:
Position 787 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTAACAAAGTCCACAAA
Hairpin Sequence:
5'-CCGG-CCTGGTAACAAAGTCCACAAA-CTCGAG-TTTGTGGACTTTGTTACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTAACAAAGTCCACAAACTCGAGTTTGTGGACTTTGTTACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTAACAAAGTCCACAAACTCGAGTTTGTGGACTTTGTTACCAGG-3'

Other clones with same target sequence:

(none)