Construct: shRNA TRCN0000084522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023434.1-458s1c1
DNA Barcode:
GTTCTCAACTTGGTTTGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tox4 (268741)
Gene Description:
TOX high mobility group box family member 4
Transcript:
RefSeq NM_023434.1 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTCAACTTGGTTTGAGTT
Hairpin Sequence:
5'-CCGG-GTTCTCAACTTGGTTTGAGTT-CTCGAG-AACTCAAACCAAGTTGAGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTCAACTTGGTTTGAGTTCTCGAGAACTCAAACCAAGTTGAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTCAACTTGGTTTGAGTTCTCGAGAACTCAAACCAAGTTGAGAAC-3'

Other clones with same target sequence:

(none)