Construct: shRNA TRCN0000084555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009056.1-1283s1c1
DNA Barcode:
GAAGCTCATCTCTCTGTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rfx2 (19725)
Gene Description:
regulatory factor X, 2 (influences HLA class II expression)
Transcript:
RefSeq NM_009056.1 (NON-CURRENT)
Match location:
Position 1283 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTCATCTCTCTGTGTAA
Hairpin Sequence:
5'-CCGG-GAAGCTCATCTCTCTGTGTAA-CTCGAG-TTACACAGAGAGATGAGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTCATCTCTCTGTGTAACTCGAGTTACACAGAGAGATGAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTCATCTCTCTGTGTAACTCGAGTTACACAGAGAGATGAGCTTC-3'

Other clones with same target sequence:

(none)