Construct: shRNA TRCN0000084585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009557.1-953s1c1
DNA Barcode:
CGGGAAGAACTTCAGCCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp46 (22704)
Gene Description:
zinc finger protein 46
Transcript:
RefSeq NM_009557.1 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGAACTTCAGCCAGAA
Hairpin Sequence:
5'-CCGG-CGGGAAGAACTTCAGCCAGAA-CTCGAG-TTCTGGCTGAAGTTCTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGAACTTCAGCCAGAACTCGAGTTCTGGCTGAAGTTCTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGAACTTCAGCCAGAACTCGAGTTCTGGCTGAAGTTCTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000331762