Construct: shRNA TRCN0000084712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033327.1-564s1c1
DNA Barcode:
CGTGGAAGATGAGTCAATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp423 (94187)
Gene Description:
zinc finger protein 423
Transcript:
RefSeq NM_033327.2 (CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGAAGATGAGTCAATTTA
Hairpin Sequence:
5'-CCGG-CGTGGAAGATGAGTCAATTTA-CTCGAG-TAAATTGACTCATCTTCCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGAAGATGAGTCAATTTACTCGAGTAAATTGACTCATCTTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGAAGATGAGTCAATTTACTCGAGTAAATTGACTCATCTTCCACG-3'

Other clones with same target sequence:

(none)