Construct: shRNA TRCN0000084713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007548.1-3347s1c1
DNA Barcode:
GCAACCTTTCTCTATGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prdm1 (12142)
Gene Description:
PR domain containing 1, with ZNF domain
Transcript:
RefSeq NM_007548.1 (NON-CURRENT)
Match location:
Position 3347 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCTTTCTCTATGATAAT
Hairpin Sequence:
5'-CCGG-GCAACCTTTCTCTATGATAAT-CTCGAG-ATTATCATAGAGAAAGGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCTTTCTCTATGATAATCTCGAGATTATCATAGAGAAAGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCTTTCTCTATGATAATCTCGAGATTATCATAGAGAAAGGTTGC-3'

Other clones with same target sequence:

(none)