Construct: shRNA TRCN0000084740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011541.3-516s1c1
DNA Barcode:
GCACTTCTGATTCTGTGCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcea1 (21399)
Gene Description:
transcription elongation factor A (SII) 1
Transcript:
RefSeq NM_011541.3 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTCTGATTCTGTGCGAT
Hairpin Sequence:
5'-CCGG-GCACTTCTGATTCTGTGCGAT-CTCGAG-ATCGCACAGAATCAGAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTCTGATTCTGTGCGATCTCGAGATCGCACAGAATCAGAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTCTGATTCTGTGCGATCTCGAGATCGCACAGAATCAGAAGTGC-3'

Other clones with same target sequence:

(none)