Construct: shRNA TRCN0000084743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175558.2-2581s1c1
DNA Barcode:
GCCTACTAACTCTGCCTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp446 (269870)
Gene Description:
zinc finger protein 446
Transcript:
RefSeq NM_175558.2 (NON-CURRENT)
Match location:
Position 2581 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACTAACTCTGCCTGTAA
Hairpin Sequence:
5'-CCGG-GCCTACTAACTCTGCCTGTAA-CTCGAG-TTACAGGCAGAGTTAGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACTAACTCTGCCTGTAACTCGAGTTACAGGCAGAGTTAGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACTAACTCTGCCTGTAACTCGAGTTACAGGCAGAGTTAGTAGGC-3'

Other clones with same target sequence:

(none)