Construct: shRNA TRCN0000084857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028665.1-1198s1c1
DNA Barcode:
GCCAACCTAGCAACTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd42 (73845)
Gene Description:
ankyrin repeat domain 42
Transcript:
RefSeq NM_028665.1 (NON-CURRENT)
Match location:
Position 1198 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACCTAGCAACTCAAGAT
Hairpin Sequence:
5'-CCGG-GCCAACCTAGCAACTCAAGAT-CTCGAG-ATCTTGAGTTGCTAGGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACCTAGCAACTCAAGATCTCGAGATCTTGAGTTGCTAGGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACCTAGCAACTCAAGATCTCGAGATCTTGAGTTGCTAGGTTGGC-3'

Other clones with same target sequence:

(none)