Construct: shRNA TRCN0000084869
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133801.1-583s1c1
- DNA Barcode:
- CCTGTACAGAACTGGTACAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gtf2f1 (98053)
- Gene Description:
- general transcription factor IIF, polypeptide 1
- Transcript:
- RefSeq NM_133801.1 (NON-CURRENT)
- Match location:
- Position 583 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGTACAGAACTGGTACAAT
- Hairpin Sequence:
-
5'-CCGG-CCTGTACAGAACTGGTACAAT-CTCGAG-ATTGTACCAGTTCTGTACAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGTACAGAACTGGTACAATCTCGAGATTGTACCAGTTCTGTACAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGTACAGAACTGGTACAATCTCGAGATTGTACCAGTTCTGTACAGG-3'
Other clones with same target sequence:
-
TRCN0000302087
