Construct: shRNA TRCN0000084989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016877.2-366s1c1
DNA Barcode:
CCCTTGGAAATAGATGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnot4 (53621)
Gene Description:
CCR4-NOT transcription complex, subunit 4
Transcript:
RefSeq NM_016877.2 (NON-CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGGAAATAGATGATATT
Hairpin Sequence:
5'-CCGG-CCCTTGGAAATAGATGATATT-CTCGAG-AATATCATCTATTTCCAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGGAAATAGATGATATTCTCGAGAATATCATCTATTTCCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGGAAATAGATGATATTCTCGAGAATATCATCTATTTCCAAGGG-3'

Other clones with same target sequence:

(none)