Construct: shRNA TRCN0000084994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201638.1-559s1c1
DNA Barcode:
CCCTAAACTTAGGGAACTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl14 (210529)
Gene Description:
methyltransferase like 14
Transcript:
RefSeq NM_201638.1 (NON-CURRENT)
Match location:
Position 559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000084994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAAACTTAGGGAACTCAT
Hairpin Sequence:
5'-CCGG-CCCTAAACTTAGGGAACTCAT-CTCGAG-ATGAGTTCCCTAAGTTTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAAACTTAGGGAACTCATCTCGAGATGAGTTCCCTAAGTTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAAACTTAGGGAACTCATCTCGAGATGAGTTCCCTAAGTTTAGGG-3'

Other clones with same target sequence:

(none)