Construct: shRNA TRCN0000085061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009329.2-954s1c1
DNA Barcode:
CATCCCTGTATAAACATCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp354a (21408)
Gene Description:
zinc finger protein 354A
Transcript:
RefSeq NM_009329.2 (NON-CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCCTGTATAAACATCTAA
Hairpin Sequence:
5'-CCGG-CATCCCTGTATAAACATCTAA-CTCGAG-TTAGATGTTTATACAGGGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCCTGTATAAACATCTAACTCGAGTTAGATGTTTATACAGGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCCTGTATAAACATCTAACTCGAGTTAGATGTTTATACAGGGATG-3'

Other clones with same target sequence:

(none)