Construct: shRNA TRCN0000085141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008736.1-441s1c1
DNA Barcode:
CCTGTCTCTATGGAAGGGCCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nrl (18185)
Gene Description:
neural retina leucine zipper gene
Transcript:
RefSeq NM_008736.1 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCTCTATGGAAGGGCCT
Hairpin Sequence:
5'-CCGG-CCTGTCTCTATGGAAGGGCCT-CTCGAG-AGGCCCTTCCATAGAGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCTCTATGGAAGGGCCTCTCGAGAGGCCCTTCCATAGAGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCTCTATGGAAGGGCCTCTCGAGAGGCCCTTCCATAGAGACAGG-3'

Other clones with same target sequence:

(none)