Construct: shRNA TRCN0000085208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146222.2-1948s1c1
DNA Barcode:
GCCTTGATATAAGCAAATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Msantd2 (235184)
Gene Description:
Myb/SANT-like DNA-binding domain containing 2
Transcript:
RefSeq NM_146222.2 (CURRENT)
Match location:
Position 1948 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGATATAAGCAAATGTA
Hairpin Sequence:
5'-CCGG-GCCTTGATATAAGCAAATGTA-CTCGAG-TACATTTGCTTATATCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGATATAAGCAAATGTACTCGAGTACATTTGCTTATATCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGATATAAGCAAATGTACTCGAGTACATTTGCTTATATCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000301975