Construct: shRNA TRCN0000085263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178761.2-2521s1c1
DNA Barcode:
CCTGTTAATCTCTACTGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp672 (319475)
Gene Description:
zinc finger protein 672
Transcript:
RefSeq NM_178761.2 (NON-CURRENT)
Match location:
Position 2521 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTAATCTCTACTGCTTT
Hairpin Sequence:
5'-CCGG-CCTGTTAATCTCTACTGCTTT-CTCGAG-AAAGCAGTAGAGATTAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTAATCTCTACTGCTTTCTCGAGAAAGCAGTAGAGATTAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTAATCTCTACTGCTTTCTCGAGAAAGCAGTAGAGATTAACAGG-3'

Other clones with same target sequence:

(none)