Construct: shRNA TRCN0000085264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178761.2-1868s1c1
DNA Barcode:
GCGATTCACCTGCATTTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp672 (319475)
Gene Description:
zinc finger protein 672
Transcript:
RefSeq NM_178761.2 (NON-CURRENT)
Match location:
Position 1868 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATTCACCTGCATTTCCAA
Hairpin Sequence:
5'-CCGG-GCGATTCACCTGCATTTCCAA-CTCGAG-TTGGAAATGCAGGTGAATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATTCACCTGCATTTCCAACTCGAGTTGGAAATGCAGGTGAATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATTCACCTGCATTTCCAACTCGAGTTGGAAATGCAGGTGAATCGC-3'

Other clones with same target sequence:

  1. TRCN0000316028