Construct: shRNA TRCN0000085291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016861.1-386s1c1
DNA Barcode:
CGTCATCCCTACAAGATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdlim1 (54132)
Gene Description:
PDZ and LIM domain 1 (elfin)
Transcript:
RefSeq NM_016861.1 (NON-CURRENT)
Match location:
Position 386 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCATCCCTACAAGATGAAT
Hairpin Sequence:
5'-CCGG-CGTCATCCCTACAAGATGAAT-CTCGAG-ATTCATCTTGTAGGGATGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCATCCCTACAAGATGAATCTCGAGATTCATCTTGTAGGGATGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCATCCCTACAAGATGAATCTCGAGATTCATCTTGTAGGGATGACG-3'

Other clones with same target sequence:

(none)