Construct: shRNA TRCN0000085301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026248.1-1548s1c1
DNA Barcode:
CGGAATCAGCTCATTTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930430A15Rik (67575)
Gene Description:
RIKEN cDNA 4930430A15 gene
Transcript:
RefSeq NM_026248.1 (NON-CURRENT)
Match location:
Position 1548 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATCAGCTCATTTCATAA
Hairpin Sequence:
5'-CCGG-CGGAATCAGCTCATTTCATAA-CTCGAG-TTATGAAATGAGCTGATTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATCAGCTCATTTCATAACTCGAGTTATGAAATGAGCTGATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATCAGCTCATTTCATAACTCGAGTTATGAAATGAGCTGATTCCG-3'

Other clones with same target sequence:

(none)