Construct: shRNA TRCN0000085320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009570.2-317s1c1
DNA Barcode:
GACCAGATTGTTGTGGAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfy1 (22767)
Gene Description:
zinc finger protein 1, Y-linked
Transcript:
RefSeq NM_009570.2 (NON-CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAGATTGTTGTGGAAGTA
Hairpin Sequence:
5'-CCGG-GACCAGATTGTTGTGGAAGTA-CTCGAG-TACTTCCACAACAATCTGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAGATTGTTGTGGAAGTACTCGAGTACTTCCACAACAATCTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAGATTGTTGTGGAAGTACTCGAGTACTTCCACAACAATCTGGTC-3'

Other clones with same target sequence:

(none)