Construct: shRNA TRCN0000085329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016851.1-1339s1c1
DNA Barcode:
CGCATGATCTATGAGATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irf6 (54139)
Gene Description:
interferon regulatory factor 6
Transcript:
RefSeq NM_016851.1 (NON-CURRENT)
Match location:
Position 1339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATGATCTATGAGATGTTT
Hairpin Sequence:
5'-CCGG-CGCATGATCTATGAGATGTTT-CTCGAG-AAACATCTCATAGATCATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATGATCTATGAGATGTTTCTCGAGAAACATCTCATAGATCATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATGATCTATGAGATGTTTCTCGAGAAACATCTCATAGATCATGCG-3'

Other clones with same target sequence:

(none)