Construct: shRNA TRCN0000085363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019935.2-2501s1c1
DNA Barcode:
CCCACGTTACTGATGTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ovol1 (18426)
Gene Description:
ovo like zinc finger 1
Transcript:
RefSeq NM_019935.2 (NON-CURRENT)
Match location:
Position 2501 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACGTTACTGATGTCATAA
Hairpin Sequence:
5'-CCGG-CCCACGTTACTGATGTCATAA-CTCGAG-TTATGACATCAGTAACGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACGTTACTGATGTCATAACTCGAGTTATGACATCAGTAACGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACGTTACTGATGTCATAACTCGAGTTATGACATCAGTAACGTGGG-3'

Other clones with same target sequence:

(none)