Construct: shRNA TRCN0000085368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173785.2-1318s1c1
DNA Barcode:
CCTACTCTTATGGGCTGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iba57 (216792)
Gene Description:
IBA57 homolog, iron-sulfur cluster assembly
Transcript:
RefSeq NM_173785.2 (NON-CURRENT)
Match location:
Position 1318 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACTCTTATGGGCTGTCTT
Hairpin Sequence:
5'-CCGG-CCTACTCTTATGGGCTGTCTT-CTCGAG-AAGACAGCCCATAAGAGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACTCTTATGGGCTGTCTTCTCGAGAAGACAGCCCATAAGAGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACTCTTATGGGCTGTCTTCTCGAGAAGACAGCCCATAAGAGTAGG-3'

Other clones with same target sequence:

(none)