Construct: shRNA TRCN0000085418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008090.3-2490s1c1
DNA Barcode:
CCCTGTAAATACAACCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gata2 (14461)
Gene Description:
GATA binding protein 2
Transcript:
RefSeq NM_008090.3 (NON-CURRENT)
Match location:
Position 2490 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTAAATACAACCTTCTT
Hairpin Sequence:
5'-CCGG-CCCTGTAAATACAACCTTCTT-CTCGAG-AAGAAGGTTGTATTTACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTAAATACAACCTTCTTCTCGAGAAGAAGGTTGTATTTACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTAAATACAACCTTCTTCTCGAGAAGAAGGTTGTATTTACAGGG-3'

Other clones with same target sequence:

(none)