Construct: shRNA TRCN0000085442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023773.1-669s1c1
DNA Barcode:
CACCTCTGAAATAATCGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mphosph8 (75339)
Gene Description:
M-phase phosphoprotein 8
Transcript:
RefSeq NM_023773.1 (NON-CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTCTGAAATAATCGGTTT
Hairpin Sequence:
5'-CCGG-CACCTCTGAAATAATCGGTTT-CTCGAG-AAACCGATTATTTCAGAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTCTGAAATAATCGGTTTCTCGAGAAACCGATTATTTCAGAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTCTGAAATAATCGGTTTCTCGAGAAACCGATTATTTCAGAGGTG-3'

Other clones with same target sequence:

  1. TRCN0000302238