Construct: shRNA TRCN0000085444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177066.2-278s1c1
DNA Barcode:
CGGTGGCAACAAGTCGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnni3k (320067)
Gene Description:
TNNI3 interacting kinase
Transcript:
RefSeq NM_177066.2 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGCAACAAGTCGCATAT
Hairpin Sequence:
5'-CCGG-CGGTGGCAACAAGTCGCATAT-CTCGAG-ATATGCGACTTGTTGCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGCAACAAGTCGCATATCTCGAGATATGCGACTTGTTGCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGCAACAAGTCGCATATCTCGAGATATGCGACTTGTTGCCACCG-3'

Other clones with same target sequence:

(none)