Construct: shRNA TRCN0000085478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008091.2-2265s1c1
DNA Barcode:
CCCTGTAATTGTTGTTTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gata3 (14462)
Gene Description:
GATA binding protein 3
Transcript:
RefSeq NM_008091.2 (NON-CURRENT)
Match location:
Position 2265 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTAATTGTTGTTTGTAT
Hairpin Sequence:
5'-CCGG-CCCTGTAATTGTTGTTTGTAT-CTCGAG-ATACAAACAACAATTACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTAATTGTTGTTTGTATCTCGAGATACAAACAACAATTACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTAATTGTTGTTTGTATCTCGAGATACAAACAACAATTACAGGG-3'

Other clones with same target sequence:

(none)