Construct: shRNA TRCN0000085537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011439.1-1962s1c1
DNA Barcode:
GCACTTGTAGATACATTCCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sox13 (20668)
Gene Description:
SRY (sex determining region Y)-box 13
Transcript:
RefSeq NM_011439.1 (NON-CURRENT)
Match location:
Position 1962 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTGTAGATACATTCCCA
Hairpin Sequence:
5'-CCGG-GCACTTGTAGATACATTCCCA-CTCGAG-TGGGAATGTATCTACAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTGTAGATACATTCCCACTCGAGTGGGAATGTATCTACAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTGTAGATACATTCCCACTCGAGTGGGAATGTATCTACAAGTGC-3'

Other clones with same target sequence:

(none)