Construct: shRNA TRCN0000085556

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029416.1-637s1c1
DNA Barcode:
CAGATGTTGCACTCCATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klf17 (75753)
Gene Description:
Kruppel-like factor 17
Transcript:
RefSeq NM_029416.1 (NON-CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085556)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGTTGCACTCCATAAAT
Hairpin Sequence:
5'-CCGG-CAGATGTTGCACTCCATAAAT-CTCGAG-ATTTATGGAGTGCAACATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGTTGCACTCCATAAATCTCGAGATTTATGGAGTGCAACATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGTTGCACTCCATAAATCTCGAGATTTATGGAGTGCAACATCTG-3'

Other clones with same target sequence:

(none)