Construct: shRNA TRCN0000085564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008602.2-1363s1c1
DNA Barcode:
GCTGCCTATGAGAGTCTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pias2 (17344)
Gene Description:
protein inhibitor of activated STAT 2
Transcript:
RefSeq NM_008602.2 (NON-CURRENT)
Match location:
Position 1363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCTATGAGAGTCTGATA
Hairpin Sequence:
5'-CCGG-GCTGCCTATGAGAGTCTGATA-CTCGAG-TATCAGACTCTCATAGGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCTATGAGAGTCTGATACTCGAGTATCAGACTCTCATAGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCTATGAGAGTCTGATACTCGAGTATCAGACTCTCATAGGCAGC-3'

Other clones with same target sequence:

(none)