Construct: shRNA TRCN0000085686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010812.1-555s1c1
DNA Barcode:
GATCCAGTTCACATCGCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxk1 (17425)
Gene Description:
forkhead box K1
Transcript:
RefSeq NM_010812.1 (NON-CURRENT)
Match location:
Position 555 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAGTTCACATCGCTATA
Hairpin Sequence:
5'-CCGG-GATCCAGTTCACATCGCTATA-CTCGAG-TATAGCGATGTGAACTGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAGTTCACATCGCTATACTCGAGTATAGCGATGTGAACTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAGTTCACATCGCTATACTCGAGTATAGCGATGTGAACTGGATC-3'

Other clones with same target sequence:

(none)